Journal: Frontiers in Microbiology

Article Title: Life in the brine of Lunenburg, Germany: unveiling microorganisms associated with Zechstein salt deposits

doi: 10.3389/fmicb.2025.1625916

Figure Lengend Snippet: Microscopic morphology of Haloarcula sp. NS06 in light (A) , scanning electron (B) and thin section electron microscopy (C,D) . (A) In a 2-days-old artificial sea water (ASW) suspension culture, single cells are pleomorphic in shape, from round to rod-like, and frequently found in short chains or small aggregates. In addition to single cells and smaller groups, larger aggregates of larger cells are visible, which we have termed “package-like aggregates.” (B) Larger assemblies of package-like aggregates and short chains of single cells on top of them are visible, if cells are cultivated on agar for 3 weeks. (C) Transmission electron microscopy of a thin section through a single rod-shaped cell of a 23-days-old suspension culture shows a dense cytoplasm containing a few circular regions of lower density and some denser structures, which most likely represent ribosomes. The cell is limited by a thin and dense layer, which is separated from the plasma by a bright appearing gap most probably representing the central part of the plasma membrane. (D) Transmission electron microscopy of a thin section of a 23-days-old suspension culture through a larger group of cells which are enclosed by fibrous sheath. The bright-appearing inclusions (arrows) are visible in most section profiles. Panel (B) shows a composite micrograph generated by merging two images acquired at different focal planes. Scale bars represent 10 μm (A) , 1 μm (B) , 0.2 μm (C) and 0.5 μm (D) .

Article Snippet: In addition, liquid cultivation was performed using five different media, which were evaluated for their ability to enrich haloarchaeal communities: artificial seawater (ASW), Marine Broth (Difco) adjusted to 195 g L –1 NaCl, R2A medium (HiMedia) at 3.12 g L –1 prepared in 100% brine, a 1:1 (v/v) mixture of brine and R2A (1.56 g L –1 ), and brine supplemented with 0.1% (w/v) yeast extract and 0.075% (w/v) casamino acids.

Techniques: Electron Microscopy, Suspension, IF-cells, Transmission Assay, Clinical Proteomics, Membrane, Generated

Journal: Frontiers in Microbiology

Article Title: Life in the brine of Lunenburg, Germany: unveiling microorganisms associated with Zechstein salt deposits

doi: 10.3389/fmicb.2025.1625916

Figure Lengend Snippet: Raman spectroscopy and colony formation on artificial sea water (ASW) media of the two isolates Haloarcula sp. NS06 and Halorubrum sp. AS12 as well as the media as a reference. Raman spectra revealed main peaks for NS06 and AS12 at 980–1,000 (double peak), 1,147 and 1,504 cm −1 , typical for carotenoids. In addition, minor peaks were recognized at 781, 828, 956, 1,048, 1,191, 1,282, 1,353, 1,389, 1,448, 1,567, and 1,664 cm –1 . The first peak can be assigned to the internal background signal from the ASW media itself, since the media is showing a similar peak at 980 cm −1 .

Article Snippet: In addition, liquid cultivation was performed using five different media, which were evaluated for their ability to enrich haloarchaeal communities: artificial seawater (ASW), Marine Broth (Difco) adjusted to 195 g L –1 NaCl, R2A medium (HiMedia) at 3.12 g L –1 prepared in 100% brine, a 1:1 (v/v) mixture of brine and R2A (1.56 g L –1 ), and brine supplemented with 0.1% (w/v) yeast extract and 0.075% (w/v) casamino acids.

Techniques: Raman Spectroscopy